N-(3-(2-(2-(3-Aminopropoxy)ethoxy)ethoxy)propyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide

N-(3-(2-(2-(3-Aminopropoxy)ethoxy)ethoxy)propyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide, a PEG-based PROTAC linker featuring an NH2 functional group, is utilized in the synthesis of PROTACs[1]. These linkers function to connect the two distinct ligands in PROTACs, specifically E3 ubiquitin ligase and the target protein[1]. PROTACs leverage the intracellular ubiquitin-proteasome system to selectively degrade target proteins, effectively attenuating tumor progression in tumor xenograft models[1]. N-(3-(2-(2-(3-Aminopropoxy)ethoxy)ethoxy)propyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide (compound 11) at concentrations of 0-2 mM for 3 hours, combined with protein G (compound 6), exhibits fluorescence under streptavidin probes. This makes N-(3-(2-(2-(3-Aminopropoxy)ethoxy)ethoxy)propyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide a valuable tool for high-throughput analysis of protein-small molecule interactions and profiling enzyme activities using a microarray constructed by surface derivatization methods[2].